66. When cleaning a horizontal laminar flow hood, which of the following is the best practice?

Answer: D

Explanation:

Avoid direct contact with the HEPA filter.

Maintaining the integrity of the HEPA filter is crucial in a laminar flow hood. Direct contact can compromise the filter's efficiency and contaminate the workspace, making it essential to avoid such contact during cleaning.

A) Spray the cleaner directly into the hood.

This practice is incorrect as spraying cleaner directly into the hood can cause aerosols that may disrupt the airflow pattern. It could lead to contamination of the workspace by dispersing particles into the air rather than effectively cleaning surfaces.

B) Clean the hood sides from bottom to top.

Cleaning from bottom to top is not advisable as it can lead to drips or contaminants running down to the already cleaned areas. The best practice is to clean from top to bottom to ensure that any particles fall downwards and are captured during the cleaning process.

C) Use a clean fabric cloth.

While using a clean cloth is generally a good practice, it is not specific enough to ensure that no lint or fibers are released during cleaning. The use of lint-free wipes is recommended to prevent contamination of the sterile environment.

D) Avoid direct contact with the HEPA filter.

Avoiding direct contact with the HEPA filter is vital to preserving its function and ensuring the safety of the clean environment. This practice prevents potential damage and contamination that could arise from improper cleaning methods.

Conclusion

The correct answer emphasizes the importance of protecting the HEPA filter to maintain the efficiency of the laminar flow hood. Other options either compromise cleaning efficacy or can introduce contaminants, making them less suitable for best practices in a sterile environment. Maintaining proper cleaning protocols ensures a safe and effective workspace for sensitive processes.